Application of SNP Probe Capture Enrichment and Massively Parallel Sequencing in the Analysis of Degraded and Mixed Samples
Samples that are highly degraded and mixed in nature are commonly found in mass disaster events, missing persons, and forensic casework and often pose issues with conventional short tandem repeat (STR) typing by capillary electrophoresis (CE). DNA fragment sizes less than 150 bp present issues as two intact primer binding sites may not be present […]
Assessing Crude Extracts from Reference Samples in Massively Parallel Sequencing Workflows
Forensic science tends to be a slow-moving field, but every so often new technology helps push forensic testing forward. Massively parallel sequencing (MPS) is one such technology with substantial potential benefit for forensic DNA testing. Through a technology transfer partnership, Ohio BCI and the Battelle Memorial Institute (Columbus, Ohio) have implemented MPS-based mitochondrial DNA (mtDNA) […]
Molecular Dissection of a Crime Scene: Introducing STR Sequencing in Routine Investigation
I currently work as a post-doctoral researcher at the Institute of Legal Medicine, Ludwig Maximilian University of Munich (LMU). My ongoing research concerns the application of NGS in the routine forensic work. Written by: Marta Diepenbroek, Institute of Legal Medicine I started to work in forensics in 2012 and I was very […]
Exploring the Value of Biogeographic Ancestry in the Absence of a DNA Database
There are many scenarios encountered in forensic casework in which bone may be the only viable sample type for DNA testing, including fires, terrorist attacks, natural disasters, war conflicts, airline crashes, homicides, human trafficking, and mass graves. In these cases, the condition of recovered human remains can range from relatively intact to highly degraded and/or […]
Is MPS Right for Your Forensics Lab?
Next-generation sequencing (NGS), or massively parallel sequencing (MPS), is a powerful tool for genomic research. This high-throughput technology is fast and accessible—you can acquire a robust data set from a single run. While NGS systems are widely used in evolutionary biology and genetics, there is a window of opportunity for adoption of this technology in […]
Molecular Autopsies in the Whole Genome Sequencing Era
Every year, nearly 8 million people die from sudden cardiac death, which is defined as the unexpected death of a seemingly healthy person due to malfunctions in the heart’s electrical system and loss of cardiac function. Although sudden cardiac death (SCD) is usually associated with mature adults, SCD claims thousands of young lives every year. In […]
Harnessing the Power of Massively Parallel Sequencing in Forensic Analysis
The rapid advancement of next-generation sequencing technology, also known as massively parallel sequencing (MPS), has revolutionized many areas of applied research. One such area, the analysis of mitochondrial DNA (mtDNA) in forensic applications, has traditionally used another method—Sanger sequencing followed by capillary electrophoresis (CE). Although MPS can provide a wealth of information, its initial […]
A Cost-effective Workflow for Massively Parallel Sequencing of Drug Metabolizing Enzymes
After taking a course in Pharmacogenomics as part of my PhD program, I found the transdisciplinary approach required to understand the subject, combining both genetics and drug response, was an interesting combination that I wanted to explore. As part of a capstone project, the opportunity to work on a project in this area presented itself, […]
Under the Microscope – Walther Parson
Unknown perpetrators of crime cannot be identified with the current forensic use of DNA. The European Horizon 2020 Project VISAGE (Visual Attributes Through Genomics) aims to overcome this limitation by developing, validating, and implementing a set of molecular genetic tools for predicting appearance, age, and ancestry from unknown trace donors directly from their traces left […]
