Home » Using Next-Generation Sequencing (NGS) to Improve Sexual Assault Investigations Workshop
Sexual assault kits make up the majority of operational casework for many laboratories in the United States. Next-generation sequencing offers enhanced technical and analytical capabilities that can aid in processing these kits effectively. This workshop explores how NGS can obtain more information, provide more investigative leads and thus reduce the backlog. A panel of experts will discuss (1) the strengths and limitations of CE and NGS, (2) the value of Y-STRs and SNPs for short and long range familial searches, (3) options for STR and SNP mixture deconvolution, and (4) data interpretation and validation studies to enable seamless implement NGS for sexual assault resolution.
Handouts include development validation studies and implementation strategies.
Lab directors, Technical Leaders, Forensic scientists, Criminalists
Time | Presentation title | Speaker |
1:00 – 1:10 | Why NGS, Why Now? Baselining sexual assault workflows in operational labs
| Bruce Budowle |
1:10 – 1:40 | A comparison of tools and technologies
| Swathi A. Kumar |
1:40 – 2:10 | Validating STRs for sexual assault: NGS vs CE | Bruce Budowle |
2:10 – 2:25 | BREAK | |
2:25 – 2:50 | Validating SNPs for identity and investigative leads
| Rachel Oefelein |
2:50 – 3:20 | Deconvoluting and interpreting mixtures with SNP and STR profiles
| June Snedecor |
3:20 – 3:50 | A cookbook for implementation and preparation for admissibility hearings
| Mandi Van Buren |
3:50 – 4:00 | Conclusion |
Sexual assault kits make up the majority of operational casework for many laboratories in the United States. Next-generation sequencing offers enhanced technical and analytical capabilities that can aid in processing these kits effectively. This workshop explores how NGS can obtain more information, provide more investigative leads and thus reduce the backlog. A panel of experts will discuss (1) the strengths and limitations of CE and NGS, (2) the value of Y-STRs and SNPs for short and long range familial searches, (3) options for STR and SNP mixture deconvolution, and (4) data interpretation and validation studies to enable seamless implement NGS for sexual assault resolution.
Handouts include development validation studies and implementation strategies.
Lab directors, Technical Leaders, Forensic scientists, Criminalists
Time | Presentation title | Speaker |
1:00 – 1:10 | Why NGS, Why Now? Baselining sexual assault workflows in operational labs
| Bruce Budowle |
1:10 – 1:40 | A comparison of tools and technologies
| Swathi A. Kumar |
1:40 – 2:10 | Validating STRs for sexual assault: NGS vs CE | Bruce Budowle |
2:10 – 2:25 | BREAK | |
2:25 – 2:50 | Validating SNPs for identity and investigative leads
| Rachel Oefelein |
2:50 – 3:20 | Deconvoluting and interpreting mixtures with SNP and STR profiles
| June Snedecor |
3:20 – 3:50 | A cookbook for implementation and preparation for admissibility hearings
| Mandi Van Buren |
3:50 – 4:00 | Conclusion |
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